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The weight of structural proof favors the idea that an ATP-dependent ligase is the ancestral state from which NAD+-dependent ligases evolved, by acquisition of the specialized structural area (Ia) that binds the NMN leaving team. All recognized NAD+-dependent ligases have domain Ia, and they can't use ATP like a substrate for ligase adenylylation, presumably for the reason that LigA enzymes deficiency “motif IV” of your OB domain, the element of ATP-dependent DNA ligases that is thought to connect with the PPi leaving group of ATP (five, 19). This “both/or” circumstance of DNA ligase substrate specificity has been roiled by many seemingly conflicting reviews concerning the nucleotide specificity of archaeal DNA ligases.

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